Neutralization of the inhibitory effect of sodium polyanetholesulfonate on Neisseria meningitidis in blood cultures processed with the Du Pont Isolator System.
AUTOR(ES)
Scribner, R K
RESUMO
The inhibitory and bactericidal effects of sodium polyanetholesulfonate on Neisseria meningitidis were neutralized by blood lysis which occurs within the pediatric Isolator 1.5 Microbial tube (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.). Lysed blood was more effective than 2.5% gelatin in preventing the inhibitory effect. All but 1 of 16 N. meningitidis strains were killed by 0.06% sodium polyanetholesulfonate in the absence of lysed blood, whereas none were killed by 1.0% sodium polyanetholesulfonate when lysed blood was present. The possible clinical relevance of these results was reflected in the improved detection of meningococcemia in children when the Isolator 1.5 Microbial tube was compared with a conventional broth system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=271241Documentos Relacionados
- Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media.
- Inhibitory effect in vitro of sodium polyanethol sulfonate on the growth of Neisseria meningitidis.
- Effect of Sodium Polyanetholesulfonate on Antimicrobial Systems in Blood
- Contamination of cultures processed with the isolator lysis-centrifugation blood culture tube.
- Effect of delays in processing on the survival of Mycobacterium avium-M. intracellulare in the isolator blood culture system.