New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.
AUTOR(ES)
Efimov, V A
RESUMO
A rapid and convenient method for the synthesis of deoxyribooligonucleotides has been developed using the phosphotriester approach. The advantage of this methodology for work in solution was successfully demonstrated in synthesis of a number of DNA fragments up to 32-long. Adaptation of the presented method to solid-phase synthesis allows a pentadecamer to be assembled in 4-5 hours using dinucleotides as coupling units.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=326957Documentos Relacionados
- Synthesis of ppTppp via phosphotriester intermediates.
- A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.
- Synthesis of 3'-phosphates of diribonucleoside monophosphates via phosphotriester intermediates.
- Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.
- Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.