New Method for Purifying Coxiella burnetii by Dextran Sulfate Precipitation Followed by Centrifugation

AUTOR(ES)

Davis, J. L.

RESUMO

This paper describes a simple method for purifying Coxiella burnetii cultivated in the chick embryo. The procedure is based upon the preliminary clarification of infected whole-egg suspensions by precipitating the low-density lipoproteins and other extraneous solids with dextran sulfate, calcium chloride, and sodium chloride solutions, and then concentrating the rickettsiae by centrifugation.

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