New method of preparing elastase toxoid from Pseudomonas aeruginosa.
AUTOR(ES)
Morihara, K
RESUMO
A new method for the preparation of elastase toxoid of Pseudomonas aeruginosa was developed. A chloroacetyl peptide derivative (CICH2CO-HOLeu-Ala-Gly-NH2; HOLeu, N-hydroxy-L-leucine), an active-site-directed irreversible inhibitor of Pseudomonas aeruginosa elastase, was used to prepare elastase toxoid with or without pretreatment with Formalin and L-lysine. Elastase toxoid thus obtained appeared to be ideal, possessing negligible enzyme activity while retaining full antigenicity and immunogenicity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=268571Documentos Relacionados
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