Nitric oxide production, inhibitory, antioxidant and antimycobacterial activities of the fruits extract and flavonoid content of Schinus terebinthifolius
AUTOR(ES)
Bernardes, Natalia R., Heggdorne-Araújo, Marlon, Borges, Isabela F. J. C., Almeida, Fabricio M., Amaral, Eduardo P., Lasunskaia, Elena B., Muzitano, Michelle F., Oliveira, Daniela B.
FONTE
Rev. bras. farmacogn.
DATA DE PUBLICAÇÃO
2014-12
RESUMO
The extract of the fruits from Schinus terebinthifolius Raddi, Anacardiaceae, was obtained by exhaustive extraction with methanol. Its fractions and isolated compounds were collected by fractionation with RP-2 column chromatography. The crude extract, the flavonoid fraction and the isolated compound identified as apigenin (1), were investigated regarding its inhibitory action of nitric oxide production by LPS-stimulated macrophages, antioxidant activity by DPPH and the antimycobacterial activity against Mycobacterium bovis BCG. The samples exhibited a significant inhibitory effect on the nitric oxide production (e.g., 1, IC50 19.23 ± 1.64 µg/ml) and also showed antioxidant activity. In addition, S. terebinthifolius samples inhibited the mycobacterial growth ( e.g., 1, IC50 14.53 ± 1.25 µg/ml). The necessary concentration to produce 50% of the maximum response (IC50) of these activities did not elicit a significant cytotoxic effect when compared with the positive control (100% of lysis). The antioxidant and nitric oxide inhibition activity displayed by S. terebinthifolius corroborates its ethnopharmacological use of this specie as an anti-inflammatory. In addition, our results suggest that the flavonoids of S. terebinthifolius are responsible for the activities found. We, describe for the first time the activity against Mycobacterium bovis BCG and the inhibition of nitric oxide production for S. terebinthifolius.
Documentos Relacionados
- Schinus terebinthifolius: phenolic constituents and in vitro antioxidant, antiproliferative and in vivo anti-inflammatory activities
- Fruit Age and Changes in Abscisic Acid Content, Ethylene Production, and Abscission Rate of Cotton Fruits
- Atividade antioxidante in vitro da fração fenólica, das oleorresinas e do óleo essencial de Pimenta rosa (Schinus terebinthifolius Raddi)
- Effect of bradykinin on nitric oxide production, urea synthesis and viability of rat hepatocyte cultures
- Antioxidant, DNA damage protective, neuroprotective, and α-glucosidase inhibitory activities of a flavonoid glycoside from leaves of Garcinia gracilis