Niveis plasmaticos de e y-tocoferol de novilhos tratados com uma unica dose de destilado da desodorização do oleo de soja (DDOS)

AUTOR(ES)
DATA DE PUBLICAÇÃO

1995

RESUMO

Effect of the treatment of steers with a single oral dose of soybean oil deodorizer distillate (SODD) on the a and γ tocopherol blood plasma concentrations, was studied SODD containing 1.95 % of α-tocopherol and 8.45 % of γ-tocopherol in doses of 0.75, 1.50 or 2.25g/kg live wt. was used to treat nine 2 yr old Nelore (Bos indicus) steers to promote increases in the plasma concentration of vitamin E with a single oral treatment. The three SODD doses would correspond to 15, 30 and 45mg/kg of α-tocopherol, plus 65, 130 and 190mg/kg γ-tocopherol, being equivalent to 41.85, 83.7 and 125.5 DI of vitamin E/kg. Plasma level increases were estimated from blood plasma samples colleted at zero time, and after 14, 23, 38, 47 and 62 hours, which were centrifuged to separate the blood plasma fraction. Plasma samples were kept frozen at -20°C until analyzed. Tocopherol analyses of the SODD and plasma consisted of n-hexane extraction, filtration, evaporation and High Performance Liquid Chromatography (HPLC) on a C 18 column, using a UV detector and Absorbance readings at 292nm. Methanol: water 97:3 was the mobile phase used The results showed that a single oral treatment of steers with SODD produced significant increases in plasma a and y tocopherol levels in the first 14 hours, with maximum absorption between 38 and 62 hours, as a function of dose and tocopherol in question. The vitamin E content increased four times, showing the high capacity of SODD as a source of vitamin E for bovines in a single oral dose with the objective of increasing vitamin E blood plasma concentrations and consequently the levels in the tissues, as well. These results represent a new technologically approach that can be used before slaughter to improve the quality and nutritional value of meat.

ASSUNTO(S)

soja vitamina e desodorização bovino

ACESSO AO ARTIGO

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