Nob1p Is Required for Cleavage of the 3′ End of 18S rRNA
AUTOR(ES)
Fatica, Alessandro
FONTE
American Society for Microbiology
RESUMO
We report the characterization of a novel factor, Nob1p (Yor056c), which is essential for the synthesis of 40S ribosome subunits. Genetic depletion of Nob1p strongly inhibits the processing of the 20S pre-rRNA to the mature 18S rRNA, leading to the accumulation of high levels of the 20S pre-rRNA together with novel degradation intermediates. 20S processing occurs within a pre-40S particle after its export from the nucleus to the cytoplasm. Consistent with a direct role in this cleavage, Nob1p was shown to be associated with the pre-40S particle and to be present in both the nucleus and the cytoplasm. This suggests that Nob1p accompanies the pre-40S ribosomes during nuclear export. Pre-40S export is not, however, inhibited by depletion of Nob1p.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=151717Documentos Relacionados
- Base Pairing between U3 Small Nucleolar RNA and the 5′ End of 18S rRNA Is Required for Pre-rRNA Processing
- ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae
- 3' truncated tRNAArg is essential for in vitro specific cleavage of partially synthesized mouse 18S rRNA.
- Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis.
- Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.