Noninvasive Measurement of Bacterial Intracellular pH on a Single-Cell Level with Green Fluorescent Protein and Fluorescence Ratio Imaging Microscopy
AUTOR(ES)
Olsen, Katja N.
FONTE
American Society for Microbiology
RESUMO
We show that a pH-sensitive derivative of the green fluorescent protein, designated ratiometric GFP, can be used to measure intracellular pH (pHi) in both gram-positive and gram-negative bacterial cells. In cells expressing ratiometric GFP, the excitation ratio (fluorescence intensity at 410 and 430 nm) is correlated to the pHi, allowing fast and noninvasive determination of pHi that is ideally suited for direct analysis of individual bacterial cells present in complex environments.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=123996Documentos Relacionados
- Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level
- Osmotic Stress Leads to Decreased Intracellular pH of Listeria monocytogenes as Determined by Fluorescence Ratio-Imaging Microscopy
- Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins
- Measurement of the Effects of Acetic Acid and Extracellular pH on Intracellular pH of Nonfermenting, Individual Saccharomyces cerevisiae Cells by Fluorescence Microscopy
- Single-cell Raman and fluorescence microscopy reveal the association of lipid bodies with phagosomes in leukocytes