Normal Serum Cytotoxicity for P32-Labeled Smooth Enterobacteriaceae II. Fate of Macromolecular and Lipid Phosphorus of Damaged Cells
AUTOR(ES)
Spitznagel, John K.
RESUMO
Spitznagel, John K. (University of North Carolina, Chapel Hill). Normal serum cytotoxicity for P32-labeled smooth Enterobacteriaceae. II. Fate of macromolecular and lipid phosphorus of damaged cells. J. Bacteriol. 91:148–152. 1966.—Fresh, normal adult guinea pig serum released micro- and macromolecular forms of metabolically incorporated P32 from smooth Escherichia coli. A significant amount of P32-labeled phospholipid was also released. The overall acid solubility distribution of the P32 label in bacterial suspensions was very little affected in fresh serum, although P32-labeled cytoplasmic contents and cell walls of E. coli lost some P32 when they were suspended in fresh or heat-inactivated normal serum. These findings confirmed and extended earlier evidence that the peripheral wall and membrane structures of smooth E. coli are extensively damaged by factors in fresh normal serum.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=315924Documentos Relacionados
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