Novel bacteriological assay for detection of potential antiviral agents.
AUTOR(ES)
Block, T M
RESUMO
A prototype assay for the initial screening of potential antiviral agents that uses bacterial growth on selective media is described. The human immunodeficiency virus (HIV) protease recognition sequence was inserted into the tetracycline resistance (Tet) protein encoded by plasmid pBR322 of Escherichia coli. Expression of both the HIV protease and the modified Tet protein prevented growth in the presence of tetracycline. However, inhibition of the HIV protease restored tetracycline resistance. Thus, potential HIV protease inhibitors can be identified by their ability to confer tetracycline resistance to this bacterial strain. The assay is simple, rapid, and inexpensive, and this concept can be applied to the search for inhibitors of other viral proteases.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=172057Documentos Relacionados
- Genetic engineering of antiviral agents.
- Virus receptors: implications for pathogenesis and the design of antiviral agents.
- ABC of AIDS. Treatment of infections and antiviral agents.
- A cytotoxicity assay for evaluation of candidate anti-Pneumocystis carinii agents.
- 9-Anilinoacridines as potential antileishmanial agents.
