Novel insertion mutation in Caenorhabditis elegans.

AUTOR(ES)

Eide, D J

RESUMO

The mutation e1662 is an allele of the Caenorhabditis elegans unc-54 gene induced with the difunctional alkylating agent 1,2,7,8-diepoxyoctane. unc-54 encodes the major myosin heavy chain isozyme of body wall muscle cells. Filter-transfer hybridization and DNA sequence analysis show that e1662 is an insertion of 288 base pairs of DNA within unc-54. The inserted DNA is identical to a 288-base pair region of unc-54 located ca. 600 base pairs from the insertion site. Thus, e1662 is a displaced duplication. A 14-base pair sequence located at one end of the duplicated segment is found adjacent to the site of insertion. These homologous sequences are juxtaposed head-to-tail by the insertion event. e1662 thus contains a tandem direct repeat extending across one of its junctions.

Documentos Relacionados

• The genome of Caenorhabditis elegans.
• Structure, evolution and properties of a novel repetitive DNA family in Caenorhabditis elegans.
• Structures of spontaneous deletions in Caenorhabditis elegans.
• The Tc2 transposon in Caenorhabditis elegans.
• Extrachromosomal DNA transformation of Caenorhabditis elegans.