Novel Method for Detection, Typing, and Quantification of Human Papillomaviruses in Clinical Samples

AUTOR(ES)

Hart, Keith W.

FONTE

American Society for Microbiology

RESUMO

We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a κ value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease.

Documentos Relacionados

• A Novel and Rapid PCR-Based Method for Genotyping Human Papillomaviruses in Clinical Samples
• Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes.
• Novel Method Using a Laser Scanning Cytometer for Detection of Mycobacteria in Clinical Samples
• Development of a Multiplex PCR Technique for Detection and Epidemiological Typing of Salmonella in Human Clinical Samples
• Detection, verification, and quantification of adverse drug reactions