Novel selection for tetracycline- or chloramphenicol-sensitive Escherichia coli.

AUTOR(ES)

Craine, B L

RESUMO

A method for selecting tetracycline- or chloramphenicol-sensitive Escherichia coli cells from a population of predominantly resistant cells is described. This method depends on the inability of drug-sensitive cells to induce lambda receptors in the presence of chloramphenicol or tetracycline, protein synthesis-inhibiting drugs. The addition of bacteriophage lambda vir to a mixture of drug-sensitive and drug-resistant cells, induced for lambda receptors in the presence of tetracycline or chloramphenicol, preferentially kills the drug-resistant cells (which are capable of inducing lambda receptors). The result is a culture enriched for the sensitive cells. Several common strains used for transformation were compared for their ability to be selected. E. coli 294 was found to be superior.

Documentos Relacionados

• Chloramphenicol-Sensitive Escherichia coli Strain Expressing the Chloramphenicol Acetyltransferase (cat) Gene
• CHLORAMPHENICOL-SENSITIVE AND -INSENSITIVE PHASES OF THE LETHAL ACTION OF STREPTOMYCIN1
• ANALYSIS OF THE CHLORAMPHENICOL-SENSITIVE AND CHLORAMPHENICOL-RESISTANT STEPS IN THE INITIATION OF DNA SYNTHESIS IN E. coli B/r
• Four codons in the cat-86 leader define a chloramphenicol-sensitive ribosome stall sequence.
• Selection for loss of tetracycline resistance by Escherichia coli.