NUCLEAR MAGNETIC RESONANCE STUDIES OF THE STRUCTURE AND BINDING SITES OF ENZYMES, X. PREPARATION OF SELECTIVELY DEUTERATED ANALOGS OF STAPHYLOCOCCAL NUCLEASE

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RESUMO

Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The 1H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonated sites.

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