Nuclease-hypersensitive sites in chromatin of the estrogen-inducible apoVLDL II gene of chicken.

AUTOR(ES)

Kok, K

RESUMO

DNAseI-hypersensitive sites were localized in apoVLDL II chromatin from chicken. In the liver two sites at 1.75 and 1.0 kb upstream from the cap-site are present before the gene is activated. After induction by estradiol a number of additional sites appear, three in the promotor region of the gene, one within the coding region and two behind the poly-A signal. These sites disappear when the expression of the gene is shut off upon estradiol withdrawal. All sites appear to be tissue-specific in that they are not found in other tissues of the rooster. However, in oviduct of the laying hen we find a hypersensitive site at 1.6 kb in front of the gene.

Documentos Relacionados

• In vivo footprinting of the estrogen-inducible vitellogenin II gene from chicken.
• Nuclease-hypersensitive sites in the chromatin domain of the chicken lysozyme gene.
• Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.
• Estrogen-inducible and liver-specific expression of the chicken Very Low Density Apolipoprotein II gene locus in transgenic mice.
• DNase I- and micrococcal nuclease-hypersensitive sites in the human apolipoprotein B gene are tissue specific.