Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).

AUTOR(ES)

Prunell, A

RESUMO

Chromatin was reconstituted from core histones and recombinant plasmid DNAs carrying poly(dA) . poly(dT) inserts of various lengths. A 97-bp insert was found to occupy discrete and regularly-spaced positions on the edges of the nucleosome. This insert cannot, however, be entirely included due to a block in the center of the particle. In contrast, nucleosomes reconstitute on a shorter 20-bp insert. In this case, the insert shows a marked preference for the edges of the particle. Possible structural and physiological implications of these observations are discussed.

Documentos Relacionados

• Transcription initiation from a poly(dA) tract.
• Complete disproportionation of duplex poly(dT)·poly(dA) into triplex poly(dT)·poly(dA)·poly(dT) and poly(dA) by coralyne
• In vitro transcription of a poly(dA) x poly(dT)-containing sequence is inhibited by interaction between the template and its transcripts.
• Vibrational normal modes and dynamical stability of DNA triplex poly(dA). 2poly(dT): S-type structure is more stable and in better agreement with observations in solution.
• Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-inserted genes.