Nucleosome Translational Position, Not Histone Acetylation, Determines TFIIIA Binding to Nucleosomal Xenopus laevis 5S rRNA Genes

AUTOR(ES)

Howe, LeAnn

FONTE

American Society for Microbiology

RESUMO

We sought to study the binding constraints placed on the nine-zinc-finger protein transcription factor IIIA (TFIIIA) by a histone octamer. To this end, five overlapping fragments of the Xenopus laevis oocyte and somatic 5S rRNA genes were reconstituted into nucleosomes, and it was subsequently shown that nucleosome translational positioning is a major determinant of the binding of TFIIIA to the 5S rRNA genes. Furthermore, it was found that histone acetylation cannot override the TFIIIA binding constraints imposed by unfavorable translational positions.

Documentos Relacionados

• Restricted Specificity of Xenopus TFIIIA for Transcription of Somatic 5S rRNA Genes
• Regular arrangement of nucleosomes on 5S rRNA genes in Xenopus laevis.
• Yeast TFIIIA + TFIIIC/tau-factor, but not yeast TFIIIA alone, interacts with the Xenopus 5S rRNA gene.
• Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo.
• DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.