Nucleotide sequence of a cyanobacterial nifH gene coding for nitrogenase reductase

AUTOR(ES)

Mevarech, Moshe

RESUMO

The nucleotide sequence of nifH, the structural gene for nitrogenase reductase (component II or Fe protein of nitrogenase) from the cyanobacterium Anabaena 7120 has been determined. Also reported are 194 bases of the 5′-flanking sequence and 170 bases of the 3′-flanking sequence. The predicted amino acid sequence was compared with that determined for the complete nitrogenase reductase of Clostridium pasteurianum and the cysteine-containing peptides of the protein from Azotobacter vinelandii. Amino acid sequences around five cysteines, located in the NH2-terminal two-thirds of the protein, are highly conserved in all three species. Codon usage in the first gene from a cyanobacterium to be sequenced shows striking asymmetries for eight amino acids.

Documentos Relacionados

• Nucleotide sequence of the R.meliloti nitrogenase reductase (nifH) gene.
• Mutations affecting regulation of the Klebsiella pneumoniae nifH (nitrogenase reductase) promotor.
• Detection and characterization of cyanobacterial nifH genes.
• Rhizobium japonicum nitrogenase Fe protein gene (nifH).
• The DNA sequence of the Rhodobacter capsulatus nifH gene.