Nucleotide sequence of the Escherichia coli mutH gene.
AUTOR(ES)
Grafstrom, R H
RESUMO
The complete nucleotide sequence of mutH gene from E. coli has been determined. Based on the deduced amino acid sequence, the MutH protein has a molecular weight of 25.4 kdaltons in agreement with the previous estimates based on SDS-polyacrylamide gel electrophoresis of the purified protein. Deletion analysis of the DNA sequences upstream of mutH has identified the promoter region for this gene. Two independently isolated temperature sensitive alleles of the mutH gene have also been sequenced. One mutation results in an amino acid change at position 27 (thr to leu) while the other occurs at position 156 (asp to asn).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=340716Documentos Relacionados
- GATC sequences, DNA nicks and the MutH function in Escherichia coli mismatch repair.
- Mutagenesis by Mutator Gene mutH1 in Continuous Cultures of Escherichia coli
- Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12.
- The function of Asp70, Glu77 and Lys79 in the Escherichia coli MutH protein
- Depletion of the cellular amounts of the MutS and MutH methyl-directed mismatch repair proteins in stationary-phase Escherichia coli K-12 cells.
