Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.
AUTOR(ES)
Zalacain, M
RESUMO
The nucleotide sequence of a 1467 bp fragment of Streptomyces hygroscopicus DNA containing the gene (hyg) encoding a hygromycin B phosphotransferase (HPH) has been determined. The N-terminal amino acid sequence of HPH determined by automated Edman degradation has allowed the coding sequence of the hyg gene to be identified. The translation initiation triplet is GTG and 5 bp preceding it there is a sequence complementary to the 3'-end of 16S rRNA from S. lividans. The transcriptional start and termination sites have been determined; the presumptive promoter region has only partial homology to that of the Streptomyces vinaceus vph gene and is different to the promoter sequences of other Streptomyces genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=339529Documentos Relacionados
- Nucleotide sequence analysis reveals linked N-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of Streptomyces hygroscopicus.
- Transcriptional regulation of bialaphos biosynthesis in Streptomyces hygroscopicus.
- Some chemical and physical characteristics of pantomycin, and antiobiotic isolated from Streptomyces hygroscopicus.
- Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus
- Nucleotide sequence of the xylose isomerase gene from Streptomyces violaceoniger.
