Observation of Cytoplasmic and Vacuolar Malate in Maize Root Tips by 13C-NMR Spectroscopy 1
AUTOR(ES)
Chang, Keejong
RESUMO
The accumulation of malate by maize (Zea mays L.) root tips perfused with KH13CO3 was followed by 13C nuclear magnetic resonance spectroscopy. In vivo nuclear magnetic resonance spectra contained distinct signals from two pools of malate in maize root tips, one at a pH ∼5.3 (assigned to the vacuole) and one at a pH > 6.5 (assigned to the cytoplasm). The ratio of cytoplasmic to vacuolar malate was lower in 12 millimeter long root tips than in 2 millimeter root tips. The relatively broad width of the signals from C1- and C4-labeled vacuolar malate indicated heterogeneity in vacuolar pH. During the 3 hour KH13CO3 treatment, 13C-malate accumulated first primarily in the cytoplasm, increasing to a fairly constant level of ∼6 millimolar by 1 hour. After a lag, vacuolar malate increased throughout the experiment.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1055819Documentos Relacionados
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