Occurrence of Isocitrate Lyase in a Thermophilic Bacillus Species
AUTOR(ES)
Daron, Harlow H.
RESUMO
A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medium with acetate as a carbon source. The specific activity was 400 times lower in extracts of organisms utilizing glucose as a carbon source. With crude extracts, enzyme activity was strongly stimulated by Mg++, but cysteine and ethylenediaminetetraacetate had little effect. It appeared to be more heat-stable than the pure isocitrate lyase from Pseudomonas indigofera.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=276498Documentos Relacionados
- Isocitrate Lyase from a Thermophilic Bacillus: Effect of Salts on Enzyme Activity
- Glyoxylate bypass enzymes in Yersinia species and multiple forms of isocitrate lyase in Yersinia pestis.
- Isocitrate Lyase in Green Leaves 1
- Production of extracellular alpha-glucosidase by a thermophilic Bacillus species.
- Fatty Acid Composition of Lipid Extracts of a Thermophilic Bacillus Species
