Occurrence of N-Formylkynurenine in Extracts of Neurospora crassa: Evidence for the Activity of Tryptophan Pyrrolase
AUTOR(ES)
Chen, James
RESUMO
N-formylkynurenine and kynurenine have been detected in extracts of tryptophan-grown Neurospora crassa. When the mycelia were grown in medium supplemented with l-[2-14C]tryptophan, the radioactivity was detected in N-formylkynurenine and N-formylanthranilic acid; with l-[β-14C]tryptophan, radioactivity was detected in N-formylkynurenine, kynurenine, kynurenic acid, and xanthurenic acid. The occurrence of N-formylkynurenine in extracts of tryptophan-grown Neurospora is interpreted as direct evidence for the activity of tryptophan pyrrolase in this organism. The presence of this enzyme was expected on the basis of several earlier studies, but its activity in vitro has so far escaped detection. The in vivo evidence presented here suggests its presence and contributes importantly to our understanding of the tryptophan-anthranilic acid cycle.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246830Documentos Relacionados
- Tryptophan Transport in Neurospora crassa: a Tryptophan-Binding Protein Released by Cold Osmotic Shock
- Carnitine biosynthesis in Neurospora crassa: enzymatic conversion of lysine to epsilon-N-trimethyllysine.
- Evidence for three differentially regulated catalase genes in Neurospora crassa: effects of oxidative stress, heat shock, and development.
- Cold-Induced Increase of Glycerol Kinase Activity in Neurospora crassa: Rapid Inactivation of the Enzyme In Vivo
- Pyrimidine Synthesis in Neurospora crassa: Regulation of Enzyme Activities
