Oligonucleotide-directed site-specific integration of high complexity libraries into ssDNA templates

AUTOR(ES)

Hale, M. B.

FONTE

Oxford University Press

RESUMO

We present an approach that generates an oligomer-based library with minimal need for restriction site modification of sequences in the target vector. The technique has the advantage that it can be applied for generating peptide aptamer libraries at sites within proteins without the need for introducing flanking enzyme sites. As an example we present a phagemid retroviral shuttle vector that can be used to achieve stable expression of the library in mammalian cells for the purpose of screening for peptides with desired biological activity.

Documentos Relacionados

• Oligonucleotide-directed site-specific mutagenesis in Drosophila melanogaster.
• Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis.
• High-efficiency oligonucleotide-directed plasmid mutagenesis.
• Oligonucleotide-directed restriction endonuclease digestion.
• Chromogenic identification of oligonucleotide-directed mutants.