Oligonucleotide mutagenesis of the lacPUV5 promoter.

AUTOR(ES)

Munson, L M

RESUMO

Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence and other promoter mutations located in this region.

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