One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis

AUTOR(ES)

Cribb, Pamela, Scapini, Juan Pablo, Serra, Esteban

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2002-09

RESUMO

Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.

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