Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology

AUTOR(ES)

Zinni, María A., Aljinovic, Ernesto M., Iglesias, Luis E., Iribarren, Adolfo M.

FONTE

Química Nova

DATA DE PUBLICAÇÃO

2004-06

RESUMO

The preparation of 2', 3'-di-O-hexanoyluridine (2) by a Candida antarctica B lipase-catalysed alcoholysis of 2', 3', 5'-tri-O-hexanoyluridine (1) was optimised using an experimental design. At 25 ºC better experimental conditions allowed an increase in the yield of 2 from 80% to 96%. In addition to the yield improvement, the volume reaction could be diminished in a factor of 5 and the reaction time significantly shortened.

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