Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS
AUTOR(ES)
Oliveira, Fabiano A. S., Madureira, Fernando D., Lopes, Renata P., Ferreira, Marina G., Soto-Blanco, Benito, Melo, Marília M.
FONTE
Quím. Nova
DATA DE PUBLICAÇÃO
2014
RESUMO
This paper describes the optimization of a multiresidue chromatographic analysis for the identification and quantification of 20 pesticides in bovine milk, including three carbamates, a carbamate oxime, six organophosphates, two strobilurins, a pyrethroid, an oxazolidinedione, an aryloxyphenoxypropionate acid/ester, a neonicotinoid, a dicarboximide, and three triazoles. The influences of different chromatographic columns and gradients were evaluated. Furthermore, four different extraction methods were evaluated; each utilized both different solvents, including ethyl acetate, methanol, and acetonitrile, and different workup steps. The best results were obtained by a modified QuEChERS method that lacked a workup step, and that included freezing the sample for 2 hours at -20 ºC. The results were satisfactory, yielding coefficients of variation of less than 20%, with the exception of the 50 µg L-1 sample of famoxadone, and recoveries between 70 and 120%, with the exception of acephate and bifenthrin; however, both analytes exhibited coefficients of variation of less than 20%.
Documentos Relacionados
- Hepatitis B e antibody determination: comparison and evaluation of four different methods.
- Comparison of C18-Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovis in Bovine Milk Samples and Analysis of Samples by PCR
- Liquid chromatographic determination of ampicillin in bovine and dog plasma by using a tandem solid-phase extraction method.
- Comparison of High-Performance Liquid Chromatographic and Microbiological Methods for Determination of Voriconazole Levels in Plasma
- Liquid chromatographic determination of amoxicillin concentrations in bovine plasma by using a tandem solid-phase extraction method.