Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1
AUTOR(ES)
Hao, Zhikui, Cai, Yujie, Liao, Xiangru, Zhang, Xiaoli, Fang, Zhiyou, Zhang, Dabing
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2012-03
RESUMO
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
Documentos Relacionados
- Factors Affecting Glycerol Production by a Newly Isolated Osmophilic Yeast1
- Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus
- 'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation
- Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum
- Thermostable chitinase from Cohnella sp. A01: isolation and product optimization