Optimization of the Interferon Assay Using Inhibition of Semliki Forest Virus-Ribonucleic Acid Synthesis

AUTOR(ES)

Koblet, Hans

RESUMO

The method for assaying chicken interferon by its inhibition of viral ribonucleic acid (RNA) synthesis was optimized for the chicken embryo fibroblast-Semliki Forest virus (SFV) system, with respect to time, multiplicity of infection, and addition of actinomycin D and 3H-uridine. Incorporation of 3H into viral RNA is reproducible, and amounts to 104 to 2 × 104 counts per min per uninhibited culture per 1 μCi per 6 hr. The assay may be carried out in less than 1 day and is sensitive (0.05 units/ml) and exact (±20% of the mean titers on different days); it can be used for purification procedures as well.

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