Oral lactate intensifies insulin toxicity during severe insulin-induced hypoglycemia in mice
AUTOR(ES)
Vilela, Vanessa Rodrigues, Antunes, Marina Masetto, Godoi, Vilma Aparecida Ferreira, Travassos, Patricia Batista, Souza, Helenir Medri de, Bazotte, Roberto Barbosa
FONTE
Braz. J. Pharm. Sci.
DATA DE PUBLICAÇÃO
26/07/2018
RESUMO
ABSTRACT We investigated whether oral lactate could prevent seizures and deaths in mice with severe hypoglycemia induced by a high dose of insulin. For this purpose, mice were fasted for 15 h and then given an intraperitoneal injection of regular insulin (5.0 U/kg or 10.0 U/kg). Immediately after insulin injection, the mice received an oral dose of saline (control), glucose (5.5 mmol/kg), or lactate (18.0 mmol/kg). Glucose and lactate levels were measured in the blood and brain before and after the seizures began. Glucose and lactate delayed (p < 0.05) the onset of seizures associated with severe insulin-induced hypoglycemia. Elevated (p < 0.05) brain levels of lactate were associated with an absence of seizures in mice that received glucose or lactate, suggesting that lactate could prevent convulsions associated with severe insulin-induced hypoglycemia. However, the same oral dose of lactate that delayed the onset of convulsions also increased the mortality rate. In contrast, diazepam (3.0 mg/kg) prevented seizures and markedly decreased the frequency of death during severe insulin-induced hypoglycemia. The results demonstrated that in contrast to oral glucose, oral lactate intensifies insulin toxicity.
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