Organização estrutural da matriz extracelular e qualificação de DNA em nucleos de celulas dos tendões calcaneus communis e Flexor Digitorum superficialis de frangos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Two tendons of chickens with 21 and 41 days old from tibiotarsus region were utilized: calcaneus communis tendon and fIexor digitorum superficialis tendon that are submitted to compressive and tensional forces, respectively. The aim of this study was to detect morphological and functional differences, related with molecular order _nd crystallinity between these two tendons. Tendons that pass above joints have fibrocartilagenous characteristics in response to compressive forces, presenting high amounts of free glycosaminoglycans and organized with the collagen bundles. Otherwise, tendons subjected to tensional forces have minor glycosaminoglycans amount, in agreement with the decreased metachromasy after toluidine blue staining and with the great values found in the intumescing tests with 3% acetic acid. Form birefringence curves analyses reveal a higher ordered aggregational state and crystallinity in this tendon according to the optical retard values. Cells of the calcaneus communis tendon exhibit great morphological variations, as its nuclei, wich have forms ranging from elongated to round, similar to chondrocytes. Its was also watched an increase on the DNA amount at these nuclei with the ages advance when compared to the amount found in chicken erythrocytes, here utilized as control for the geometric and absorciometric parameter. However, ali these data indicate that these tendons, for needing constant adaptative processes, have a high organization and orientation of their components, besides a high metabolic activity of the cells

ASSUNTO(S)

colageno matriz extracelular tendões

ACESSO AO ARTIGO

Documentos Relacionados