Osmotic Stress Leads to Decreased Intracellular pH of Listeria monocytogenes as Determined by Fluorescence Ratio-Imaging Microscopy

AUTOR(ES)

Fang, Weihuan

FONTE

American Society for Microbiology

RESUMO

Intracellular pH (pHi) of Listeria monocytogenes was determined after exposure to NaCl or sorbitol in liquid and solid media (agar). Both compounds decreased pHi, and recovery on solid medium was impaired compared to that in liquid medium. N,N′-dicyclohexylcarbodiimide abolished pHi recovery, and lowering aw with glycerol showed no effect on pHi.

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