Osmotically Sensitive Brucella in Infected Normal and Immune Macrophages
AUTOR(ES)
McGhee, Jerry R.
RESUMO
When Brucella suis is grown in tissue cultures of normal guinea pig macrophages, the Brucella multiplies significantly without the induction of osmotically sensitive forms. In immune macrophages in the presence of normal guinea pig serum, there is a reduction in the number of intracellular Brucella and no multiplication is seen over a 72-hr period. After 6 hr of exposure to immune macrophages, however, approximately 50% of the surviving intracellular Brucella are osmotically sensitive, i.e., they will survive and grow only on medium containing 0.2 m sucrose. Brucella grown in immune macrophages, in the presence of rabbit antiserum against whole guinea pig serum, show the presence of osmotically sensitive forms, although at a reduced level compared to the number seen with immune macrophages in normal guinea pig serum.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=415869Documentos Relacionados
- Medium-Dependent Activity of Immune Serum on Brucella-Infected Macrophages
- Ribonucleic acid synthesis in normal and immune macrophages after antigenic stimulus.
- Localization of brucella antigens that elicit a humoral immune response in Brucella abortus-infected cells.
- Expression and Bactericidal Activity of Nitric Oxide Synthase in Brucella suis-Infected Murine Macrophages
- VARIATIONS IN NORMAL AND BRUCELLA-IMMUNE RABBIT SERUM AS DETERMINED BY PAPER-PARTITION CHROMATOGRAPHY
