Overmethylation of DNAs by the EcoRI methylase.
AUTOR(ES)
Berkner, K L
RESUMO
EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI endonuclease activity, and thus is presumably at or near the EcoRI sequences (5') NAATTN.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341993Documentos Relacionados
- Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites.
- Specificity of substrate recognition by the EcoRI restriction endonuclease.
- Does the specific recognition of DNA by the restriction endonuclease EcoRI involve a linear diffusion step? Investigation of the processivity of the EcoRI endonuclease.
- Sequences spanning the EcoRI substrate site.
- Cleavage of single stranded oligonucleotides by EcoRI restriction endonuclease.
