Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity.
AUTOR(ES)
Uhlin, B E
RESUMO
Plasmid pBEU14, which carries the Escherichia coli recA+ gene and which can be amplified by manipulation of growth temperature, was constructed. When pBEU14 deoxyribonucleic acid was amplified, a high rate of synthesis and accumulation of recA protein resulted. Amplification of the recA gene and protein did not cause induction of prophage lambda, indicating that the proteolytic activity of the recA protein was not stimulated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=216207Documentos Relacionados
- Cobalt: an effector of E. coli recA protein activity.
- RecA protein of Escherichia coli has a third essential role in SOS mutator activity.
- Inactivation of prophage in ultraviolet-irradiated Escherichia coli: dependence on recA gene activity.
- Evidence that the recA441 (tif-1) mutant of Escherichia coli K-12 contains a thermosensitive intragenic suppressor of RecA constitutive protease activity.
- UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA.