Ozone Alteration of Membrane Permeability in Chlorella: I. Permeability of Potassium Ion as Measured by 86Rubidium Tracer 1

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The addition of ozone to a suspension of Chlorella sorokiniana causes a rapid loss of K+, as measured by efflux of 86Rb from prelabeled cells. The efflux of the tracer is stimulated some 15 to 20 times over that of the control. For about 100 microliters per liter ozone, about 25 minutes (6 × 10−8 moles O3 delivered per minute) of exposure are required for a 50% depletion of the intracellular K+. The stimulation of K+ efflux is nearly linearly dependent upon the amount of ozone delivered into the solution. Following short pulses of ozone (lasting 1 to 5 minutes), efflux rates return to the control level but only after about 15 minutes.

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