P Element-Mediated in Vivo Deletion Analysis of White-Apricot: Deletions between Direct Repeats Are Strongly Favored
AUTOR(ES)
Kurkulos, M.
RESUMO
We have isolated and characterized deletions arising within a P transposon, P[hsw(a)], in the presence of P transposase. P[hsw(a)] carries white-apricot (w(a)) sequences, including a complete copia element, under the control of an hsp70 promoter, and resembles the original w(a) allele in eye color phenotype. In the presence of P transposase, P[hsw(a)] shows a high overall rate (approximately 3%) of germline mutations that result in increased eye pigmentation. Of 234 derivatives of P[hsw(a)] with greatly increased eye pigmentation, at least 205 carried deletions within copia. Of these, 201 were precise deletions between the directly repeated 276-nucleotide copia long terminal repeats (LTRs), and four were unique deletions. High rates of transposase-induced precise deletion were observed within another P transposon carrying unrelated 599 nucleotide repeats (yeast 2μ FLP; recombinase target sites) separated by 5.7 kb. Our observation that P element-mediated deletion formation occurs preferentially between direct repeats suggests general methods for controlling deletion formation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1205858Documentos Relacionados
- Vectors for P element-mediated gene transfer in Drosophila.
- Repression of P Element-Mediated Hybrid Dysgenesis in Drosophila Melanogaster
- Interaction of the Enhancer of White-Apricot with Transposable Element Alleles at the White Locus in Drosophila Melanogaster
- Highly Selective Actions of HuR in Antagonizing AU-Rich Element-Mediated mRNA Destabilization
- Partial Revertants of the Transposable Element-Associated Suppressible Allele White-Apricot in Drosophila Melanogaster: Structures and Responsiveness to Genetic Modifiers