p21-Activated Kinase 1 Plays a Critical Role in Cellular Activation by Nef
AUTOR(ES)
Fackler, Oliver T.
FONTE
American Society for Microbiology
RESUMO
The activation of Nef-associated kinase (NAK) by Nef from human and simian immunodeficiency viruses is critical for efficient viral replication and pathogenesis. This induction occurs via the guanine nucleotide exchange factor Vav and the small GTPases Rac1 and Cdc42. In this study, we identified NAK as p21-activated kinase 1 (PAK1). PAK1 bound to Nef in vitro and in vivo. Moreover, the induction of cytoskeletal rearrangements such as the formation of trichopodia, the activation of Jun N-terminal kinase, and the increase of viral production were blocked by an inhibitory peptide that targets the kinase activity of PAK1 (PAK1 83-149). These results identify NAK as PAK1 and emphasize the central role its kinase activity plays in cytoskeletal rearrangements and cellular signaling by Nef.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=85477Documentos Relacionados
- Nef Associates with p21-Activated Kinase 2 in a p21-GTPase-Dependent Dynamic Activation Complex within Lipid Rafts
- Regulation of Macropinocytosis by p21-activated Kinase-1
- Human immunodeficiency virus type 1 Nef associates with a member of the p21-activated kinase family.
- Human Immunodeficiency Virus Type 1 Nef Activates p21-Activated Kinase via Recruitment into Lipid Rafts
- GIT1 Activates p21-Activated Kinase through a Mechanism Independent of p21 Binding