Paper Disk-Agar Diffusion Assay of Penicillin in the Presence of Streptomycin
AUTOR(ES)
Raahave, Dennis
RESUMO
Microbiological assay of individual antibiotics in mixtures of antibiotics depends on the use of selective inactivation and/or of test bacteria with differential susceptibility. Controlled experiments revealed that streptomycin in concentrations of 20 and 40 μg/ml did not influence a disk diffusion assay of penicillin with Sarcina lutea (ATCC 9341) as the test organism. In the case of penicillin concentrations less than or equal to 1 IU/ml, addition of 80 μg of streptomycin per ml influenced the penicillin assay significantly. Clinical use of streptomycin resulting in levels above 40 μg/ml usually did not occur; therefore penicillin could be assayed as though streptomycin were not present. We observed additionally that S. lutea was unable to grow on agar plates prepared with semicarbazide hydrochloride.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=444700Documentos Relacionados
- Quantitation of Imidazoles by Agar-Disk Diffusion
- Disk Agar Diffusion Susceptibility Testing of Yeasts
- Assay of Streptomycin by the Paper-Disc Plate Method
- Correlation of Netilmicin Agar Dilution and Disk Diffusion Susceptibilities
- Mezlocillin Susceptibility Tests by the Single-Disk Agar Diffusion Method