Partial purification and characterization of human gamma (immune) interferon.

AUTOR(ES)

Yip, Y K

RESUMO

Human gamma (immune) interferon (IFN-gamma) was produced in lymphocyte cultures stimulated with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate) and purified phytohemagglutinin. Physicochemical analysis showed that human IFN-gamma is a glycoprotein with an isoelectric point around 8.6 and an apparent molecular weight of 58,000 +/- 3000. A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. This purification process resulted in an increase in specific activity from about 10(4) (crude culture fluid) to an estimated 10(7) units per mg of protein with a cumulative recovery of about 40% of the IFN activity.

Documentos Relacionados

• Purification of two subspecies of human gamma (immune) interferon.
• Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.
• Purification and partial characterization of human lymphoblast interferon.
• Partial purification and characterization of RTG-2 fish cell interferon.
• Large-scale production and physicochemical characterization of human immune interferon.