Partial Substitution of Serum in Hematopoietic Cell Line Media by Synthetic Polymers
AUTOR(ES)
Mizrahi, A.
RESUMO
Several synthetic polymers (hydroxyethyl starch, sodium carboxymethyl cellulose, polyvinylpyrrolidone) markedly improved the growth of three human lymphocyte cell lines [Roswell Park Memorial Institute (RPMI) 1348, 1788, and 8098]. Growth was stimulated when each of these polymers was added to RPMI 1640 medium supplemented with only 2% fetal bovine serum. Dextran T-40, T-70, and T-110 varied in their effect on the growth of these cell lines. Dextran T-250 and Haemaccel did not improve cell yields when partially substituted for the serum. The successful partial substitution of polymers for serum was specific for individual cell lines.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=376821Documentos Relacionados
- Production of Tetracycline by Streptomyces aureofaciens in Synthetic Media
- Role of Sodium Carboxymethyl Cellulose and Hydroxyethyl Starch in Hematopoietic Cell Line Cultures
- In vitro generation of hematopoietic stem cells from an embryonic stem cell line.
- Total Substitution of Bromodeoxyuridine for Thymidine in the DNA of a Bromodeoxyuridine-Dependent Cell Line
- Nonmyristoylated Abl proteins transform a factor-dependent hematopoietic cell line.
