Participation of ABF-1 protein in expression of the Saccharomyces cerevisiae CAR1 gene.

AUTOR(ES)

Kovari, L Z

RESUMO

DNA fragments previously shown to be required for expression of the CAR1 (arginase) gene in Saccharomyces cerevisiae and to support transcriptional activation of a reporter gene in a heterologous expression vector were shown to bind purified regulatory protein ABF-1. Two ABF-1 sites were identified in the CAR1 upstream region, one to which ABF-1 protein bound with high affinity and a second to which it bound much less avidly. The higher-affinity ABF-1 binding site upstream of CAR1 was an effective competitor of the HMRE, ARS1 B domain, and COR2-GFI binding sequences for protein binding. Point mutations in the CAR1 high-affinity ABF-1 binding site resulted in a 12-fold loss of transcriptional activation of a reporter gene compared with the wild-type CAR1 DNA fragment. These data are consistent with the suggestion that ABF-1 protein is one of the transcription factors involved in expression of the CAR1 gene.

Documentos Relacionados

• Participation of RAP1 protein in expression of the Saccharomyces cerevisiae arginase (CAR1) gene.
• Isolation of the CAR1 Gene from Saccharomyces cerevisiae and Analysis of Its Expression
• Participation of the yeast activator Abf1 in meiosis-specific expression of the HOP1 gene.
• Disruption of the CAR1 Gene Encoding Arginase Enhances Freeze Tolerance of the Commercial Baker's Yeast Saccharomyces cerevisiae
• Combinatorial regulation of the Saccharomyces cerevisiae CAR1 (arginase) promoter in response to multiple environmental signals.