Pasteurella multocida Gene Expression in Response to Iron Limitation
AUTOR(ES)
Paustian, Michael L.
FONTE
American Society for Microbiology
RESUMO
Pasteurella multocida is the causative agent of a wide range of diseases in avian and mammalian hosts. Gene expression in response to low iron conditions was analyzed in P. multocida using whole-genome microarrays. The analysis shows that the expression of genes involved in energy metabolism and electron transport generally decreased 2.1- to 6-fold while that of genes used for iron binding and transport increased 2.1- to 7.7-fold in P. multocida during the first 2 h of growth under iron-limiting conditions compared with controls. Notably, 27% of the genes with significantly altered expression had no known function, illustrating the limitations of using publicly available databases to identify genes involved in microbial metabolism and pathogenesis. Taken together, the results of our investigations demonstrate the utility of whole-genome microarray analyses for the identification of genes with altered expression profiles during varying growth conditions and provide a framework for the detailed analysis of the molecular mechanisms of iron acquisition and metabolism in P. multocida and other gram-negative bacteria.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=98476Documentos Relacionados
- Transcriptional Response of Pasteurella multocida to Nutrient Limitation
- Transcriptional Response of Pasteurella multocida to Defined Iron Sources
- Pulmonary response to intratracheal challenge with Pasteurella haemolytica and Pasteurella multocida.
- Immune response to dna vaccine expressing transferrin binding protein a gene of Pasteurella multocida
- Serological Response to Pasteurella multocida NanH Sialidase in Persistently Colonized Rabbits