Pathogenesis of Clostridium botulinum Type A: Study of In Vivo Toxin Release by Implantation of Diffusion Chambers Containing Spores, Vegetative Cells, and Free Toxin
AUTOR(ES)
Suzuki, J. B.
RESUMO
Millipore diffusion chambers (MDC) with 0.22-μm filters loaded with spores or vegetative cells of Clostridium botulinum were surgically implanted intraperitoneally (ip) into guinea pigs. MDC expose C. botulinum spores or vegetative cells to body fluids yet protect them from phagocytes. Guinea pigs receiving MDC containing 109 spores plus 108 polymorphonuclear (PMN) leukocytes and MDC with 109 vegetative cells died within 48 hr, indicating that toxin was released and diffused out. MDC with 109 spores alone allowed 90% of animals to survive for at least 96 hr. Microscopically, it was observed that vegetative cells in MDC were disintegrated and leukocytes plus spores were phagocytized and germinated; spores alone remained intact and phase bright. Chemotactic attraction of leukocytes to MDC walls was also observed. Apparently, body fluids do not attack spores; thus, PMN leukocyte engulfment is essential for germination and release of spore-bound toxin in this type of C. botulinum pathogenesis. However, vegetative cells appear to be attacked by bacteriolytic enzymes (e.g., lysozyme) in body fluids, and leukocyte engulfment is not essential for toxin release.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=416213Documentos Relacionados
- ENZYMES OF GLUCOSE AND PYRUVATE CATABOLISM IN CELLS, SPORES, AND GERMINATED SPORES OF CLOSTRIDIUM BOTULINUM1
- Inactivation of Vegetative Cells, but Not Spores, of Bacillus anthracis, B. cereus, and B. subtilis on Stainless Steel Surfaces Coated with an Antimicrobial Silver- and Zinc-Containing Zeolite Formulation
- Rapid Detection of Clostridium botulinum Toxin by Capillary Tube Diffusion
- Heat Injury and Recovery of Vegetative Cells of Clostridium botulinum Type E
- Cellular Responses of Mice to Diffusion Chambers I. Reactions to Intraperitoneal Diffusion Chambers Containing Listeria monocytogenes and to Bacteria-Free Chambers