Pattern of Wnt ligand expression during chick eye development
AUTOR(ES)
Rossi, E., Siwiec, F., Yan, C.Y.I.
FONTE
Brazilian Journal of Medical and Biological Research
DATA DE PUBLICAÇÃO
31/07/2007
RESUMO
The dorsoventral axis of the eye is determined prior to optic cup invagination. A variety of signaling pathways have been implicated in the maintenance of the optic dorsoventral axis, including, but not limited to, bone morphogenetic protein 4, Sonic Hedgehog and retinoic acid. Here, we investigated the possible contribution of Wnt ligands to the establishment or maintenance of the optic axis by analyzing their expression pattern during early chick optic development. We performed in situ hybridization of Wnt-1, Wnt-3a, Wnt-4, and Wnt-5a during the optic vesicle, early optic cup and established optic cup stages and focused our analysis on the optic region. Our data showed that Wnt-5a, but none of the others, is expressed in the dorsal region of the eye starting from the Hamburger and Hamilton stage 14 (HH14). These results are supported by cryosections of the labeled optic region, which further reveal that Wnt-5a is expressed only in the dorsal retinal pigmented epithelium. Thus, we propose that Wnt-5a is a marker for dorsal retinal pigmented epithelium in chick embryos from HH14 to HH19.
Documentos Relacionados
- Expression of pax-6 during urodele eye development and lens regeneration.
- Temporal reiteration of a precise gene expression pattern during nematode development.
- Temporal and Spatial Expression Pattern of Sucrose Synthase during Tomato Fruit Development.
- Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum
- Functional expression of A-currents in embryonic chick sympathetic neurones during development in situ and in vitro.