PCR-based immortalization and screening of hierarchical pools of cDNAs.

AUTOR(ES)

D'Esposito, M

RESUMO

Starting from sequences of at least 60 bp, PCR-based screening has been developed to recover cDNAs from libraries without the necessity for hybridization or extensive DNA extraction steps. The method maintains the indefinite availability of even scarce cDNA libraries and provides an estimate of the relative abundance of the mRNA species. Isolation of a cDNA clone can be done in less than a week. cDNAs were isolated that were cognate for fragments of expressed sequences and for an exon predicted from genomic sequence.

Documentos Relacionados

• A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes.
• Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of northern blot for affinity capturing of cDNAs.
• A PCR-based method for high stringency screening of DNA libraries.
• Isolation and characterization of three soybean extensin cDNAs.
• Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs