PCR hot start using primers with the structure of molecular beacons (hairpin-like structure)
AUTOR(ES)
Kaboev, O. K.
FONTE
Oxford University Press
RESUMO
A new technique of PCR hot start using oligonucleotide primers with a stem–loop structure is developed here. The molecular beacon oligonucleotide structure without any chromophore addition to the ends was used. The 3′-end sequence of the primers was complementary to the target and five or six nucleotides complementary to the 3′-end were added to the 5′-end. During preparation of the reaction mixture and initial heating, the oligonucleotide has a stem–loop structure and cannot serve as an effective primer for DNA polymerase. After heating to the annealing temperature it acquires a linear structure and primer extension can begin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=113163Documentos Relacionados
- Fingerprinting genomes using PCR with arbitrary primers.
- Multiplex detection of four pathogenic retroviruses using molecular beacons
- Structure–function relationships of shared-stem and conventional molecular beacons
- An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose.
- Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR