Penetration of Rhizopus oligosporus into Soybeans in Tempeh

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Histological observations were made on the penetration of hyphae of Rhizopus oligosporus into soybean cotyledons in tempeh, an Indonesian soybean food. Hyphal penetrations averaged one per 1,400 μm2 (±390 μm2) on the curved (outer) cotyledon surface and one per 1,010 μm2 (±340 μm2) on the flat (inner) one. Hyphae infiltrated to a depth of 742 μm, or about 25% of the average width of a soybean cotyledon. This previously unreported degree of penetration offers partial explanation for the rapid physical and chemical changes in soybeans during tempeh fermentation.

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