Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis

AUTOR(ES)

Camargo, JB, Langoni, H, Troncarelli, MZ, Machado, JG, Lucheis, SB, Padovani, CR

FONTE

Journal of Venomous Animals and Toxins including Tropical Diseases

DATA DE PUBLICAÇÃO

2010

RESUMO

Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR.

Documentos Relacionados

• Comparison of PCR with direct examination of bone marrow aspiration, myeloculture, and serology for diagnosis of visceral Leishmaniasis in immunocompromised patients.
• Evaluation of PCR for diagnosis of visceral leishmaniasis.
• Convenience of Serum for Visceral Leishmaniasis Diagnosis by PCR
• Detection of Leishmania infantum in Dogs by PCR with Lymph Node Aspirates and Blood
• Detection of canine visceral leishmaniasis by conjunctival swab PCR